Table 1 Primers and probes used in the study.

From: A preliminary study of the use of MinION sequencing to specifically detect Shiga toxin-producing Escherichia coli in culture swipes containing multiple serovars of this species

E. coli strain

Target gene

Primer/probe sequence 5’–3’

bp*

PCR E (%)

References

STEC O145:H28

stx2

F: GGG CAG TTA TTT TGC TGT GGA

R: GAA AGT ATT TGT TGC CGT ATT AAC GA

P: ATG TCT ATC AGG CGC GTT TTG ACC ATC TT

131

96

19

ehxA

F: GTG TCA GTA GGG AAG CGA ACA

R: ATC ATG TTT TCC GCC AAT G

P: CGT GAT TTT GAA TTC AGA ACC GGT GG

125

108

20

EPEC O21

wzxO21

F: CTG CTG ATG TCG CTA TTA TTG CTG

R: TGA AAA AAA GGG AAA CAG AAG AGC C

209

95

21

EAEC O104: H4

wzxO104

F: TGTCGCGCAAAGAATTTCAAC

R: AAAATCCTTTAAACTATACGCCC

P: TTGGTTTTTTTGTATTAGCAATAAGTGGTGTC

100

102

22

ExPEC O6:H31

cnf1

F: AGCGTGCAATCTATCCGTATTT

R: TGGAATTTCCCCAGTATAGGTG

173

89

23

  1. *bp: amplicon size in base pairs, PCR E (%): PCR efficiency. F: forward primer, R: reverse primer, P: probe.
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