Figure 2 | Scientific Reports

Figure 2

From: Insulin-like growth factor binding protein-3 induces senescence by inhibiting telomerase activity in MCF-7 breast cancer cells

Figure 2

IGFBP-3 increases the fraction of non-cycling cells. (A) The percentage of non-cycling cells was determined using the BrdU–Hoechst quenching technique in the presence or absence of siRNA:IGFBP-3. (B) The percentage of non-cycling cells increased from 1.25% in the absence of ponasterone A to 4.8% in its presence (p < 0.005). The percentage of non-cycling cells in the culture was reduced from 4.8 to 2.13% by co-treatment with siRNA:IGFBP-3 (p < 0.01), but not by scrambled siRNA. a. p < 0.05, ponasterone A-untreated cells vs. ponasterone A-treated cells; b. p < 0.05, ponasterone A-treated cells vs. ponasterone A-treated cells with siRNA:IGFBP-3; c. p < 0.05, ponasterone A-treated cells with scrambled siRNA vs. ponasterone A-treated cells with siRNA:IGFBP-3. Multiple comparisons were performed using one-way ANOVA with Bonferroni post-hoc analysis. Each bar represents the mean ± SD of three independent experiments.

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