Figure 2

Induction of the knockout (KO) of Pten and Arid1a in peritoneal endometriosis. (A) Schema of the experiment. Yellow dashed circles indicate peritoneal cystic lesions that formed in this experiment. KO: knockout, DOX: oral administration of doxycycline to induce KO. (B) Microscopic photographs of the peritoneal cystic lesions from each donor type by hematoxylin & eosin (HE) staining and immunostaining for Pax8, Pten, and Arid1a (200×). Epithelial cells from all donor types are positive for Pax8. Cells from Control donor mice (white arrows) express both Pten and Arid1a. They show no morphological changes with single-layer growth without stratification and nuclear atypia. Cells from iAD donor mice (gray arrows) lack Arid1a expression, but show no morphological changes. Cells from iPD donor mice (black arrows) form focal lesions with stratification and mild to moderate nuclear atypia, corresponding to atypical endometriosis. In this lesion, cells lack the expression of Pten. Cells from iPAD donor mice (yellow arrows) form focal lesions with significant stratification, papillary growth, cribriform patterns, and prominent nuclear atypia corresponding to endometrioid carcinoma. In this lesion, cells lack the expression of both Pten and Arid1a. (C) The graph shows the ratio of lesion classifications formed by the knockout (KO) induction of Pten and Arid1a by the administration of doxycycline (DOX) in the peritoneal cystic lesions of each donor origin. Cysts from Control and iAD show no morphological changes (No atypia). All cysts (100%, 8/8) from iPD are classified as atypical endometriosis (Atypical). Approximately 50% of cysts (42%, 5/12) from iPAD are classified as endometrioid carcinoma (Carcinoma) and the others as atypical endometriosis (58%, 7/12).