Table 1 Homology reduction methods and numbers of sequences used by available software. Abbreviations: HR - homology reduction, HP - homology partitioning, TM - thylakoid membrane, TL - thylakoid lumen, IM - inner membrane, OM - outer membrane. *Authors report that the threshold was set to 25% but they use data from MultiP-Schlo created with 40% cutoff.

From: Prediction of protein subplastid localization and origin with PlastoGram

Software

Year

Homology reduction strategy (cutoff)

Final number of sequences

Envelope

Stroma

TM

TL

Plastoglobule

SubChlo

2009

CD-HIT HR (60%)

262

40

49

129

44

-

ChloroRF

2010

CD-HIT HR (60%)

261

40

49

128

44

-

SubIdent

2011

CD-HIT HR (60%)

262

40

49

129

44

-

BS-KNN

2012

CD-HIT HR (60%)

253

40

46

127

40

-

ChloPred

2013

PISCES HR (25%)

259

77

60

103

19

-

SCLAP

2013

PISCES HR (60%)

341

118

72

111

41

-

WS-LCHI

2014

CD-HIT HR (60%)

262

40

49

129

44

-

MultiP-Schlo

2015

CD-HIT HR (40%)

578

199

105

233

34

30

LNP-Chlo

2016

CD-HIT HR (40%*)

578

199

105

233

34

30

SChloro

2016

HP with PSI-BLAST

367

71 (47 IM, 24 OM)

119

131

37

32

EnTrans-Chlo

2017

CD-HIT HR (40%*)

578

199

105

233

34

30

Bankapur &Patil

2022

CD-HIT HR (40%)

578

199

105

233

34

30