Table 2 Quantitative descriptors, namely velocity, turning angles, elongations, area and perimeter/area ratio of each subpopulation compared to the whole population of WC256 cells.

From: Impact of elastic substrate on the dynamic heterogeneity of WC256 Walker carcinosarcoma cells

Parameter

Subpopulation

Substrate

Median

Q1

Q3

IQR

Statistical difference

Velocity (µm/min)

Mesenchymal

Glass

0.614

0.372

0.963

0.591

***

PA

0.518

0.289

0.989

0.700

Polygonal and bigonal

Glass

0.509

0.311

0.795

0.484

–

PA

0.507

0.306

0.797

0.492

Amoeboid

Glass

1.325

0.795

2.186

1.391

***

PA

1.536

0.899

2.717

1.818

All subpopulations together

Glass

0.607

0.364

0.988

0.624

***

PA

0.655

0.365

1.287

0.922

Turning angles (°)

Mesenchymal

Glass

−15.4

−75.7

28.6

104.2

–

PA

−11.8

−68.9

34.4

103.3

Polygonal and bigonal

Glass

−37.9

−126.1

39.8

166.0

–

PA

−28.2

−121.3

41.2

162.5

Amoeboid

Glass

−10.4

−49.1

21.7

70.9

** 

PA

−5.3

−29.6

16.6

46.2

All subpopulations together

Glass

−18.6

−91.2

30.2

121.4

**

PA

−15.4

−75.7

28.6

104.2

Elongation (normalized)

Mesenchymal

Glass

0.365

0.258

0.493

0.235

***

PA

0.267

0.169

0.359

0.190

Polygonal and bigonal

Glass

0.615

0.484

0.700

0.216

***

PA

0.580

0.445

0.681

0.236

Amoeboid

Glass

0.357

0.245

0.462

0.217

***

PA

0.385

0.287

0.480

0.193

All subpopulations together

Glass

0.436

0.302

0.601

0.299

***

PA

0.427

0.288

0.585

0.297

Area (µm2)

Mesenchymal

Glass

478.9

401.5

566.0

164.5

***

PA

338.3

274.6

381.1

106.5

Polygonal and bigonal

Glass

503.9

448.9

568.2

119.3

***

PA

399.3

323.1

466.3

143.3

Amoeboid

Glass

321.4

290.9

399.3

108.4

***

PA

252.8

226.1

284.9

58.8

All subpopulations together

Glass

478.3

401.0

558.4

157.4

***

PA

326.3

259.9

414.0

154.2

Perimeter/area (1/µm2)

Mesenchymal

Glass

0.142

0.134

0.153

0.019

***

PA

0.149

0.141

0.162

0.021

Polygonal and bigonal

Glass

0.158

0.148

0.170

0.022

***

PA

0.168

0.157

0.183

0.026

Amoeboid

Glass

0.163

0.157

0.171

0.014

***

PA

0.180

0.169

0.191

0.022

All subpopulations together

Glass

0.150

0.138

0.162

0.024

***

PA

0.169

0.155

0.184

0.029

  1. Number of stars marks the statistical significance calculated by Mann–Whitney test (– for no difference * for p < 0.05, ** for p < 0.01, *** for p < 0.001).
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