Figure 3
Increased T cell-mediated immune responses by rAd5-Gn priming and Gn protein boosting. Six-week-old C57BL/6 mice (n = 5/group) are immunized intramuscularly with 1 × 108 infectious units of rAd5-Gn or alum and RNA-adjuvanted Gn protein at 0 and 2 weeks. Mice are sacrificed one week after boosting. (a, d, g), and (j) Representative flow cytometry plots showing (a) follicular helper T cells, (d) effector T cells, central memory t cells, (g) interferon γ cytokine-producing T cells, (j) activated T cells. Frequencies of (b) CXCR5, (c) PD-1 expression in CD4+ T cell, and ICOS expression in CD4 + PD-1 + populations from immunized mice spleen. Frequencies of (e) effector memory T cells and (f) central memory T cells in CD4+ T cells were analyzed using flow cytometry. IFN-γ-producing (h) CD4+ and (i) CD8+ T cells in the spleen were analyzed using flow cytometry. (k) CD69 and (l) CD25 expression in CD4 + T cells in the spleen was analyzed using flow cytometry. Data are presented as mean values ± SD. P values are calculated using one-way ANOVA with Bonferroni multiple comparison test.
