Figure 5

IPGTT and IPITT on high fat fed mice. 2-month-old mice were divided into four groups and fed either chow (standard diet; SD) or a high-fat diet (HFD) for twelve weeks (n = 18 SD WT, 18 SD Tg^sAnk1.5/+, 18 HFD WT, 20 HFD Tg^sAnk1.5/+). (a) IPGTT of 2-month-old WT and Tg^sAnk1.5/+ mice before starting a high-fat diet (n = 36 WT and 38 Tg^sAnk1.5/+). (b) IPGTT of WT and Tg^sAnk1.5/+ mice at the end of the high-fat diet period. (c) IPITT of WT and Tg^sAnk1.5/+ mice at the end of the high-fat diet period. (d) areas under the curves (AUC ± SD) calculated from glycemic curves at 2 months of age reported in (a). € AUC ± SD calculated from glycemic curves of chow (SD)- and high fat (HFD) fed mice at the end of the high-fat diet period reported in (b). (f) AUC ± SD calculated from the IPITT experiment reported in (c). * and ^# refer to WT SD vs WT HFD and Tg^sAnk1.5/+ SD vs Tg^sAnk1.5/+ HFD, respectively; * and ^#, ** and ^##, ***, **** and ^####p < 0.05, < 0.01, < 0.001, < 0.0001, respectively; n.s. = not significant. (g) representative western blot analysis of AKT phosphorylation at serine 473 in the gastrocnemius of chow-fed (SD) and high-fat diet-fed (HFD) WT and Tg^sAnk1.5/+ mice. (h) Densitometric analysis of p-AKT^Ser473 and total AKT, using actin (ponceau S staining) as a normalizer, for both dietetic regimens. Data are expressed as pAKT⁴⁷³/total AKT ratio ± SD relative to WT diet-matched mice. Densitometric analysis of sAnk1.5 signal intensity relative to WT diet-matched mice is also reported. *p < 0.05, Student’s t-test. Original blots/gel are reported in Supplementary Fig. S3 online.