Figure 7
From: Skeletal muscle overexpression of sAnk1.5 in transgenic mice does not predispose to type 2 diabetes
SERCA1 and p-AKT analysis. Ca2+-dependent ATPase activity of SERCA1a in 3-month-old (a) and 14-month-old (b) gastrocnemius muscles excised from WT and TgsAnk1.5/+ mice. Specific ATPase activity as a function of [Ca2+] is reported as a percentage of WT Vmax. Error bars represent standard deviation. Km and Vmax of the enzymatic reaction are reported next to the enzymatic activity curves. (c) Representative western blot analyses of SERCA protein levels in microsomes prepared from the gastrocnemius of 3- and 14-month-old WT and TgsAnk1.5/+. Densitometric analysis of SERCA signal intensity in 3- and 14-month-old mice was performed using Calsequestrin1 as a normalizer. p = * < 0.05, Student’s t-test. Original blots/gel are presented in Supplementary Fig. S5 online. (d) Relative phospholamban (Pln) and sarcolipin (Sln) mRNA expression by quantitative RT-PCR analysis in gastrocnemius (G), extensor digitorum longus (E) and soleus (S) muscles from WT and TgsAnk1.5/+. Cardiac mRNA (H) was used as positive control. In both graphs, Pln and Sln mRNA levels are reported as fold increase ± SD relative to WT gastrocnemius.
