Figure 4
From: Multimodal imaging of laser-induced choroidal neovascularization in pigmented rabbits
Histological and immunohistochemical analysis: (a) and (c) H&E staining images of control and treated groups, respectively. The H&E staining of the control group shows major retinal layers including the internal limiting membrane (ILM), nerve fiber layer (NFL), ganglion cell layer (GCL), inner plexiform layer (IPL), inner nuclear layer (INL), outer plexiform layer (OPL), external limiting membrane (ELM), photoreceptors (P), retinal pigment epithelium (RPE), and choroid layer (C). The retinal architecture and cells display normal morphology. In the treated group, there were significant changes in the retinal architecture (blue arrows). Scar tissue and cells were found in the treated area along with the development of neovascularization (yellow arrows). Black arrows indicate the location of choroidal vessels. Note that no retinal detachment was seen at the treated lesion. (b) and (d) corresponding immunostaining images with alpha SMA antibody of control and treated tissues. A monolayer of RPE was seen in the control group. In the treatment group, the RPE layer was discontinuous and CNV grew from the choroid layer and invaded the RPE layer and subretinal space through ruptured Bruch’s membrane (red arrows).
