Figure 1 | Scientific Reports

Figure 1

From: Prenatal exposure to benzo[a]pyrene depletes ovarian reserve and masculinizes embryonic ovarian germ cell transcriptome transgenerationally

Figure 1

Effects of F0 dam exposure to BaP on germ cell numbers in gonads of F1 embryos (Experiment 1). Pregnant F0 OG2 females were exposed to 0.033, 0.2, or 2 mg/kg-day BaP or vehicle from E6.5–11.5, and F1 embryo gonads were collected at E13.5 for germ cell counts. Graphs show means ± SEM of total number of germ cells per gonad. Dots represent values for individual embryos. (A) The number of ovarian germ cells was significantly decreased in BaP-exposed compared to oil controls (P = 0.100, effect of BaP dose, linear regression; *P = 0.014, 2 versus 0 mg/kg-day BaP, t-test). (B) The number of F1 testicular germ cells was not affected by F0 BaP exposure (P = 0.687, effect of BaP dose, linear regression). N = 2–7 F1 embryos/group, each from independent F0 dam.

Back to article page