Figure 3

Effects of F0 dam exposure to BaP during the mitotic or meiotic windows of ovarian development on ovarian follicle counts in their F2 and F3 offspring (Experiment 2). Pregnant C57BL/6J females were exposed to BaP or oil vehicle from E6.5–11.5 (mitotic developmental window) or E12.5–17.5 (predominantly meiotic developmental window). Adult, 4 to 5 month old F2 female offspring were euthanized on the day of estrus of the estrous cycle based on vaginal cytology (N = 4–6 F2 females derived from 4–5 F0 females/group). F3 female offspring were euthanized on the day of first vaginal estrus (puberty) for ovarian follicle counts (N = 9–11 F3 females derived from 5 to 7 F0 dams/group). Graphs show estimated marginal means ± SEM of total number of follicles of different developmental stages per gonad from GEE models. Dots represent values for individual females. There were no statistically significant effects of F0 BaP exposure, developmental window, or interaction between BaP and developmental window on primordial (A,D), primary (A,D), secondary (B,E), healthy antral (C,F), or atretic antral (C,F) follicle numbers in F2 or F3 ovaries.