Figure 1
mRNA expression and uncleaved protein levels of non-canonical pyroptosis-related genes are increased in siABCA1 podocytes. (a) mRNA levels of non-canonical pyroptosis-related genes in siCO and siABCA1 podocytes. (b) Protein levels of uncleaevd or pro- form of non-canonical pyroptosis-related genes in siCO and siABCA1 podocytes. The bands indicated with *(around 35 kD) in the FL-GSDMD blot are nonspecific bands. The uncropped blots are in the Supplementary Fig. S1a–c. (c) Protein levels of pro-IL1b in cell lysates and supernatants of siCO and siABCA1 podocytes. N = 3 for each group except for IL1β mRNA, which is N = 4. The uncropped blots are in the Supplementary Fig. S1a,d.
