Figure 2

Representative images of male and female mice from both the glycerol and saline groups. (a) Picrosirius red + fast green, (b) ORO, (c) H&E. n = 1–2 sections per muscle. For (c) black solid arrow represents central nuclei indicative of regeneration (only some are highlighted); white solid arrow represents a mononucleated inflammatory cell; black solid brace represents small rounded fibres indicating de-/regeneration; black arrow with yellow outline represents muscle replacement by fibrotic tissue. Note that arrows and braces are representative, and that these features are widely apparent throughout the section. Scale bars = 100 µm.