Figure 2
From: Caste-specific expressions and diverse roles of takeout genes in the termite Reticulitermes speratus

Quantitative real-time PCR expression levels (mean ± S.D., biological triplicates) of RsTO1 in head (left) and thorax and abdomen (right) among primary reproductives, workers and soldiers. Total RNA was extracted from three individuals per sample in each colony, and three biological samples (derived from different colonies) were prepared. Each value is normalized to the expression levels of GstD1 (Supplementary Table S3). Black and grey columns indicate males and females, respectively. Statistical results of the two-way ANOVA are described in each box (*P < 0.05, **P < 0.01). Different letters above the bars indicate significant differences (two-way ANOVA followed by Tukey’s test, P < 0.05).