Figure 2 | Scientific Reports

Figure 2

From: Acute exposure to organophosphorus pesticide metabolites compromises buffalo sperm function and impairs fertility

Figure 2

Protein tyrosine phosphorylation. Three major fluorescent patterns viz. non-fluorescent spermatozoa i.e. non-phosphorylated (NP), sperm bearing signal at equatorial region and mid-piece (EM) and spermatozoa with a signal at the acrosomal region, equatorial region and mid-piece (AEM).were observed during immunocytochemistry using a monoclonal anti-phosphotyrosine antibody (P1869; Sigma). The buffalo spermatozoa were exposed to 0.5, 1, and 2 μM of OPPMs viz. Omethoate (A), Paraoxon methyl (B), and TCPy (C) for 2 h.

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