Figure 5

Effect of T. boudieri and T. claveryi extracts on the mRNA expression of TNF-α and IL-6 in LPS/IFN-γ-stimulated RAW 264.7 cells. RAW 264.7 cells were stimulated with LPS/IFN-γ (100 ng/10 U/mL) and co-incubated with T. boudieri and T. claveryi extracts at concentrations of 5, 10, and 20 µg/mL for 6 h. SFN (1 µM) was used as a positive control. TNF-α (A) and IL-6 (B) mRNA expression levels were analyzed by qPCR. Data are expressed as mean ± SEM (n = 3). Statistical significance was calculated by one-way ANOVA followed by Student–Newman–Keuls post-hoc test. $P < 0.05 versus control. *P < 0.05 versus LPS/IFN-γ.