Table 2 Primers used for this study.
Primer name | Target | Use | Sequence |
|---|---|---|---|
AB0001 | pCOOFY50 backbone | RP for pCoofy50 | GGGCCCCTGGAACAGAACTTCCA |
AB0002 | pCOOFY50 backbone | FP for pCoofy50 | CGCCATTAACCTGATGTTCTGGGG |
AB0009 | DcsE from S. lavendulae | FP for DcsE | aagttctgttccaggggcccAGGGAATTCATACCCCCGG |
AB0010 | DcsE from S. lavendulae | RP for DcsE | agaacatcaggttaatggcGTCAGGCGACGATCGCCA |
ASB001 | PCS2 + FLAG-GFP backbone | FP for pCS2 + backbone | AGGCGCGCCGATTATAAAG |
ASB002 | PCS2 + FLAG-GFP backbone | RP for pCS2 + backbone | CATGGTGGCGAATTCGAATC |
ASB011 | DcsE from pCOOFY50 vector | FP for DcsE | gattcgaattcgccaccatgAGGGAATTCATACCCCCGG |
ASB012 | DcsE from pCOOFY50 vector | RP for DcsE | tctttataatcggcgcgcctGGCGACGATCGCCAGGAA |
