Figure 5
Effects of chloroquine dosing on hematocrit and parasitemia of cells in 4-organ systems. (a, b) Hematocrit in both 3D7 and W2 4-organ systems was maintained between 2 and 5% with the addition of fresh, uninfected erythrocytes every 48 h. (c) Similar to the effects observed in the single-organ dosing experiments, acute exposure of 3D7 systems to chloroquine resulted in a decrease in parasitemia by 12 h post-dose. This decrease in parasitemia appears to be semi-dose dependent with complete recrudescence in these systems by the 132-h mark. (d) Parasitemia in W2 4-organ systems did not decrease below controls until 48 h post-dose and achieved complete recrudescence by hour 132. Black lines indicate grouped parasitemia that falls below control baseline. The yellow line indicates that for hours 12 to 60, 3D7 parasitemia is statistically distinct from W2 parasitemia with a P ≤ 0.05. Data was normalized to control at each timepoint, data points are mean ± SEM. n = 12; P values = *≤ 0.05, **≤ 0.01.
