Figure 7

Functional validation of EBV- and influenza A-specific TCRs identified by RAPTER. TCR sequences identified in Fig. 6 that correspond to influenza A epitope (GILGFVFTL HTO84) and EBV BMLF1 epitope (GLCTLVAML, HTO83) were cloned and functionally tested in a Jurkat-TCR luciferase reporter assay. (A) Luciferase activity in Jurkat-TCR cells expressing Influenza M (GILGFVFTL) or EBV BMLF1 (GLCTLVAML) epitope-reactive TCRs. The relative abundance of each TCR from the RAPTER assay is indicated and the specific clone frequency is provided under the TCR name. Luciferase activity was measured in Jurkat-TCR cultured alone or Jurkat-TCR co-cultured with dendritic cells pulsed with indicated peptides. (B) Luciferase activity in Jurkat-TCR cells engineered with two non-specific background TCRs that were associated with multiple HTO at low frequencies.