Table 1 List of organisms used for in vitro testing of silvestrol sensitivity (* motif inferred from 100% consensus among 32 fruit flies across five genera).
From: Broad anti-pathogen potential of DEAD box RNA helicase eIF4A-targeting rocaglates
Species | Predicted sensitivity | Rocaglate-binding aa pattern | Measured sensitivity (S—sensitive; R—resistant) | Assay |
|---|---|---|---|---|
Schistosoma mansoni | Sensitive | TPFFQI | S | Motility and egg production assays |
Anastrepha suspensa* [AsE01] | TPYFQV | S | Cell proliferation assay | |
Drosophila melanogaster [S2] | S | Cell proliferation assay | ||
Plasmodium falciparum [3D7] | S | Viability assay in erythrocytes | ||
Aedes aegypti [Aag2] | TPHFQV | S | Cell proliferation assay | |
Toxoplasma gondii | S | Proliferation assay in MARC145 monkey kidney cells | ||
Trypanosoma brucei brucei [Lister 427] | TPVFQI | S | Viability assay | |
Leishmania amazonensis [LV78] | Resistant | TPSFQI | R | β-lactamase assay in J774 macrophage cells |
Caenorhabditis elegans | TPGFQV | R | Developmental and lifespan assays |
