Figure 4
From: NCBP1 enhanced proliferation of DLBCL cells via METTL3-mediated m6A modification of c-Myc
NCBP1 enhanced the proliferation of DLBCL cells via METTL3. (A) METTL3 expression in DLBCL tumors and normal tissues from the GEPIA database. (B) METTL3 expression in 50 DLBCL tissues and 14 inflammatory lymph nodes measured by RT‒qPCR. (C) Representative images show the differential expression of METTL3 in DLBCL tissue and inflammatory lymph nodes by immunohistochemistry (IHC). (D) Overall survival of patients grouped by METTL3 expression in DLBCL patients, high (red) or low (blue) METTL3. (E,F) The efficiency of METTL3 in lentiviral transduction SU-DHL-4 cells was confirmed by RT‒qPCR and western blotting of RNA and proteins. (G,H) Cell viability of SU-DHL-4 cells following transfection was determined by CCK8 assay at the indicated time points. (I) The proliferation of SU-DHL-4 cells following transfection was evaluated by EdU. The data are presented as the mean ± SD. Statistically significant differences were defined as follows: *P < 0.05; **P < 0.01, ***P < 0.001.
