Table 1 Determination of median lethal concentration (LC₅₀) of manganese (Mn) and manganese nanoparticles (Mn-NPs) alone and in combination with temperature (34 °C) exposed to Pangasianodon hypophthalmus for a period of 96 h.

Exposure (h)	R² value	LC₅₀ (mg/L)	95% confidence interval		S-value	Safe level (C)	Intercept	Slope
Exposure (h)	R² value	LC₅₀ (mg/L)	Lower	Higher	S-value	Safe level (C)	Intercept	Slope
Manganese exposure (Mn)
24	0.67	115.18	113.69	123.67	1.02	34.88	− 72.13	0.67
48	0.69	114.10	112.87	121.15			− 74.94	0.70
72	0.73	112.83	111.72	116.17			− 77.74	0.73
96	0.79	111.75	110.05	112.95			− 84.40	0.80
Manganese (Mn) and high temperature exposure (34 °C)
24	0.79	114.73	113.43	120.80	1.01	33.33	− 79.33	0.74
48	0.77	113.51	112.37	119.48			− 74.46	0.70
72	0.67	112.21	110.07	115.99			− 66.06	0.63
96	0.82	110.76	105.56	111.88			− 72.53	0.70
Manganese nano-particles (Mn-NP)
24	0.88	96.64	95.02	105.96	1.03	29.35	− 56.77	0.64
48	0.85	95.47	94.28	100.45			− 68.37	0.77
72	0.77	94.68	93.59	99.80			− 64.47	0.74
96	0.76	93.81	92.55	98.86			− 57.53	0.67
Manganese nano-particles (Mn-NP) and high temperature (34 °C)
24	0.88	95.36	94.39	98.18	1.01	28.03	− 98.20	0.90
48	0.88	94.71	93.80	97.32			− 97.40	0.90
72	0.91	93.77	92.96	95.28			− 104.0	0.97
96	0.72	92.39	90.77	93.30			− 95.26	0.90

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