Figure 4

Comparisons of the amounts of phosphorylated ERK1/2 (pERK), ERK, phosphorylated Akt (pAkt), and Akt among canine histiocytic sarcoma (CHS) cell lines and peripheral blood monocytes (PBMoC). (a) The representative result of Western blot. This figure was prepared by cropping the images of the bands indicating each antigen from the figures shown in Additional file 1: Fig. S5. (b) The result of statistical comparison by density analysis for Western blot in triplicate. The amounts of pERK and pAkt were significantly higher in CHS cell lines than PBMoC. **P = 0.0044.