Figure 1
From: Optimizing intra-arterial hypothermia scheme for acute ischemic stroke in an MCAO/R rat model
General experimental outlines and procedures in this study. (a) Schematics of time points for a surgical procedure, sample collection, and measurement endpoints (Created with BioRender.com). (b) Anatomical schematic of surgical procedures for MCAO (Created with BioRender.com). i: Anatomy of rat's brain arteries; ii: The ECA was ligated and cut open, and a silicone-tipped monofilament was then inserted towards the ECA; iii: The ECA was inverted parallel to the ICA, and monofilament inserted into the ICA; iv: The monofilament was carefully inserted from the ICA to the beginning of the MCA and fixed. (c) Laser Doppler detection of the brain blood flow changes of MCAO/R models. The homodynamic criteria for successful modeling were a decrease in the blood supply area of the middle cerebral artery to less than 20% of the preoperative cortical blood flow rate after the insertion of the threaded plug; If the cortical blood flow rate was restored to 80% or more of the preoperative blood flow rate when the threaded plug was removed 2 h after operative, which indicated that the reperfusion was successful. (d) Schematics of the protocol of IAH (Created with BioRender.com). A 1.5 F microcatheter, guided with a 0.010-inch Micro-guidewire, was inserted into the ICA about 0.8–1.2 cm through the ECA stump. The Micro-guidewire was withdrawn, and the other side of the microcatheter was connected to the syringe pump, which continuously pumped cold NS at a preset rate, temperature. e Anatomical Schematic of the incision location for the laser Doppler probe and measuring Tb. L1: The incision site for the laser Doppler probe (bregma/lateral: −2/6 mm (Paxinos and Watson, 2005)); L2: The incision site for measuring Tb (bregma/lateral/depth: −3/3/−2 mm (Paxinos and Watson, 2005)). Notes: CCA, common carotid artery; IAH, Intra-arterial therapeutic hypothermia; MCAO/R, middle cerebral artery occlusion/reperfusion. ICA: internal carotid artery; ECA: external carotid artery; ACA: anterior cerebral artery; MCA: middle cerebral artery; PCA: posterior cerebral artery; PPA: Pterygopalatine artery; OA: occipital artery.
