Figure 3
From: Optimizing intra-arterial hypothermia scheme for acute ischemic stroke in an MCAO/R rat model
Effects of IAH on the MCAO/R rats in the H1–H9 subgroups. (a) The representative images for TTC staining of brain tissue. (b) Ratios of the cerebral infarction volume to total brain tissue volume in TTC staining. The red parts of the brain tissue reflected normal tissue; the white parts represented cerebral infarction tissue. The ratio of infarct volume in each subgroup ranged from 23.58 to 33.42%. The H9 subgroup had the smallest proportion of cerebral infarction volume (23.58 ± 2.10%) but had no statistical differences compared with the H2 and H3 subgroup, respectively (Ni = 7, Pi > 0.05, i = 2, 3, By Dunnett test; * the reference subgroup). (c) The cerebral water content in each IAH subgroup. The H9 subgroup had the better cerebral water content (80.32 ± 0.18%) but had no significant difference with that of the H2 subgroup, respectively (Ni = 7, Pi > 0.05, i = 2, By Dunnett test; * the reference subgroup). (d) The neurological function (mNSS) in each IAH subgroup. The H9 subgroup had the better mNSS (7.57 ± 1.72) but had no significant difference with that of the H1-H5 subgroup, respectively ( Ni = 7, Pi > 0.05, i = 1–5, By Dunnett test; * the reference subgroup). Notes: IAH: Intra-arterial therapeutic hypothermia; T: temperature; t; time; Tb, ischemic brain tissue local temperature (the solid line in blue); MCAO/R: middle cerebral artery occlusion and reperfusion; TTC: 2,3, 5-Triphenyltetrazolium chloride; mNSS: modified Neurological Severity Score; TH: therapeutic hypothermia; H1: 4 °C,1/3 RICA, 30 min; H2: 4 °C, 1/2 RICA, 20 min; H3: 4 °C, 2/3 RICA, 10 min; H4: 10 °C, 2/3 RICA, 20 min; H5: 10 °C,1/2 RICA, 30 min; H6: 10 °C, 1/3 RICA, 10 min; H7:15 °C, 1/3 RICA, 20 min; H8:15 °C,1/2 RICA,10 min; H9: 15 °C, 2/3 RICA, 30 min; RICA: blood flow rate of the internal carotid artery (ICA) per minute.
