Table 1 The three factors and three levels orthogonal table (L9(34)) for IAH in MCAO/R rats.

From: Optimizing intra-arterial hypothermia scheme for acute ischemic stroke in an MCAO/R rat model

Subgroup (randomized)

Factor A: Perfusate temperature (℃)

Factor B: Infusion flow rate (RICA*, ml/min)

Factor C: Infusion duration (min)

Total infusion volume (ml)

H1

(1) 4

(1) 1/3, 0.25

(3) 30

7.5

H2

(1) 4

(2) 1/2, 0.38

(2) 20

7.5

H3

(1) 4

(3) 2/3, 0.50

(1) 10

5.0

H4

(2) 10

(3) 2/3, 0.50

(2) 20

10.0

H5

(2) 10

(2) 1/2, 0.38

(3) 30

11.3

H6

(2) 10

(1) 1/3, 0.25

(1) 10

2.5

H7

(3) 15

(1) 1/3, 0.25

(2) 20

5.0

H8

(3) 15

(2) 1/2, 0.38

(1) 10

11.3

H9

(3) 15

(3) 2/3, 0.50

(3) 30

15.0

  1. IAH intra-arterial cerebral hypothermia, MCAO/R middle cerebral artery Occlusion and Reperfusion, Ni the number of subjects in each subgroup(I = 1–9); H1–9 represent 9 subgroups of orthogonal design with different combinations of the 3 factors and 3 levels: Level 1 represents 4 °C, 1/3 RICA, and 10 min; Level 2 represents 10 °C, 1/2 RICA, and 20 min; and Level 3 represents 15 °C, 2 /3 RICA, and 30 min in the factors of perfusate temperature, infusion flow rate, and infusion duration. *RICA, blood flow rate of internal carotid artery (ICA) per minute, which was estimated as 0.75 ml/min referred previous reports21.
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