Figure 1

Plasma miRNA profile by next-generation sequencing (NGS) in the discovery cohort. (A) Volcano plot showing the 589 variables detected by NGS. Orange dots (up) refer to overexpressed miRNAs and dark blue dots (down) to downregulated miRNAs according to the differential expression criteria described (log2FC greater than 1 and less than − 1, with a p value less than 0.05). (B) 3D-principle component analysis (3D-PCA) of circulating miRNA expression in the discovery cohort. 3D-PCA decomposition of the 22 differential expressed miRNAs could distinguish most diabetes (purple) cases from the control (green) group in the NGS discovery cohort. (C) Heatmap representation of the expression levels of the 22 differential miRNAs expressed in log₂CPM. (D) KEGG dotplot showing pathway terms with p value less than 0.05 for genes that are predicted in miRNet as controlled by upregulated (UP) and downregulated (DOWN) miRNAs.