Figure 6
From: Streptozotocin induces renal proximal tubular injury through p53 signaling activation
Pharmacological inhibition of p53 signaling and SGLT2 ameliorated STZ-induced DNA damage in vivo. (a) Experimental scheme. Pifithrin-α was administered at the same time as STZ (150 mg/kg). Dapagliflozin was administered 2 h before STZ. Mice were sacrificed 24 h after the administration of STZ. N = 5 to 11 mice per group. (b) Blood glucose levels were similar among all experimental groups. (c) Plasma insulin levels were higher in STZ-treatment groups. (d) BUN levels did not differ among experimental groups. (e) Microscopic images of kidney tissue immunostained with γH2AX. (f) Separate quantification of nuclear γH2AX-positive cells in the kidney cortex and medulla. (g) Representative images of the comet assay of kidney cells isolated from mice and (h) a quantitative analysis (alkaline comet: n = 100 each, neutral comet: n = 50 each). (i) Western blots of kidney tissue lysates for γH2AX, phospho-p53, and GAPDH. Representative images of n = 3. (j) The optical densities of γH2AX and phosphor-p53 bands were normalized against that of GAPDH. In all groups, data are the means ± SEM. Statistical analyses were performed using Dunnett's post hoc test was used for multiple comparisons. * p < 0.05, Bar = 50 μm in (e).
