Figure 4
From: Using machine learning to detect coronaviruses potentially infectious to humans
Multiple sequence alignment for phylogenetically related viruses at the RBM. Multiple sequence alignment was performed with MUSCLE68 and produce visualizations with Jalview69. A darker shade shows residues conserved in at least 50% of the sequences. (a) Comparison of viruses related to Bt133 within the Merbecovirus subgenus. Ty-HKU4 and MERS are viruses known to bind human receptor hDPP4. Experimental studies found no evidence of binding from HKU5 to hDPP4. Bt133 conserves all contact residues used by Ty-HKU4 to bind hDPP4 in 24 (marked with a pink asterisk). MERS uses four of the same contact residues36 than Ty-HKU4 (indicated by a blue triangle). HKU5, the only virus in the list unable to bind hDPP4, does not share any of the 8 contact residues from Ty-HKU4, and it shows several deletions at the RBM. (b) Comparison of viruses related to LYRa3 within the Sarbecovirus subgenus. LYRa11 is phylogenetically related to SARS-CoV and there is experimental evidence of binding from both to human receptor hACE2. LYRa11 conserves 12 (out of 17) of the contact residues used by SARS-CoV29, 31 (marked with a pink asterisk). At the RBM, LYRa3 differs from LYRa11 only at H441, which is not a contact residue used by SARS-CoV. Experimental studies found no evidence of binding from ZC45 to hACE2. ZC45 conserves only 2 out of the 17 contact residues from SARS-CoV, and it shows several deletions at the RBM.
