Table 1 The candidate reference genes, the target gene, the stem loop primers, the probes, the forward primers, and the PCR efficiency.
RNA | RNA sequence | Stem loop primer | Probe | Forward primer | E (%) | R2 |
|---|---|---|---|---|---|---|
miR-193-3p | UACUGGCCUGCUAAGUCCCAAG | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCTTGGG | (FAM)ATACGACCTTGGGACT(MGB) | CGTGTACTGGCCTGCTAAGT | 113.3 | 0.997 |
miR-971-5p | CACUCUAAGCUCGAACACCAAGC | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGCTTGG | (FAM)ATACGACGCTTGGTG(MGB) | GCGTCACTCTAAGCTCGAACA | 118.0 | 0.992 |
miR-3049-5p | UCGGGAAGGCAGUUGCGGCGGACU | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAGTCCG | (FAM)ATACGACAGTCCGCC(MGB) | TCGGGAAGGCAGTTGCG | 100.5 | 0.991 |
miR-216-5p | AAAUAUCAGCUGGUAAUUCUGA | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTCAGAA | (FAM)TGGATACGACTCAGAAT(MGB) | CCGAGCGAAATATCAGCTGGTAA | 114.5 | 0.999 |
miR-7-3p | CAAGAAAUCACUCAUCUUCCU | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAGGAAG | (FAM)TGGATACGACAGGAAG(MGB) | CGACGCCAAGAAATCACTCAT | 106.4 | 0.995 |
novel-m0649-3p | CCUGUAUAUGGUCACUCUCCU | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAGGAGA | (FAM)TGGATACGACAGGAGAG(MGB) | CGTGGAGCCTGTATATGGTCAC | 110.2 | 0.995 |
miR-2788-3p | CAAUGCCCUUGGAAAUCCCA | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTGGGAT | (FAM)TGGATACGACTGGGATT(MGB) | CGAGGTCAATGCCCTTGGAA | 112.2 | 0.985 |
U6 | ACUAAAAUUGGAACGAUACAGAGAAGAUUAGCAUGGCCCCUGCGCAAGGAUGACACGCAAAAUCGUGAAGCGUUCCACAUUUUU | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAAAAAT | (FAM)CTGGATACGACAAAAATG(MGB) | GCAAAATCGTGAAGCGTTCCA | 104.0 | 0.995 |
let-7-3p | CUGUACAACUUGCUAACUUUCC | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGGAAAG | (FAM)TGGATACGACGGAAAGT(MGB) | TGCGACCTGTACAACTTGCTAA | 105.6 | 0.995 |
