Figure 6

Empagliflozin reduced arrhythmogenic events in cardiomyocytes derived from human iPSCs. Action potentials were recorded from spontaneously beating cardiomyocytes derived from human iPSCs using the intracellular microelectrodes technique. The occurrence of arrhythmogenic events (irregular and/or DAD-like events) of beating cells was assessed. Representative recordings show a normal beating rhythm (top) and irregular beating pattern (bottom) of cells treated with vehicle (right) or EMPA (left) under normoxia (A) and hypoxia (B) conditions. The black arrows indicate DAD-like events. (C) The proportion of cells showing arrhythmogenic events. Number of cells with arrhythmogenic events (total cells): normoxia, vehicle n = 6²¹ and EMPA n = 1¹⁴; hypoxia, vehicle n = 5¹² and EMPA n = 2¹⁵. P values were calculated using Fisher's exact test. **P < 0.01 and ***P < 0.001.