Figure 5
From: GPX4 overexpressed non-small cell lung cancer cells are sensitive to RSL3-induced ferroptosis
Knockdown of GPX4 (siGPX4) had an effect similar to RSL3 treatment. (A) Cell proliferation examined by cell counting. (B) LIP level measured by the fluorescence intensity of FerroOrange. (C) GSH/GSSH ratio was determined by an EZ-Glutathione assay kit. (D) Cellular ROS determined using DCFA/H2DCFDA-cellular ROS assay kit by confocal microscope (scale bar: 10 µm). (E) GPX4 expression detected by western blotting. *p < 0.05, **p < 0.01, ***p < 0.001 compared with control.
