Figure 4
Spatial expression patterns of DpBra2 during embryonic development. (a) Broad expression domain at the end of gastrulation. White arrowhead points to the opening in the centre of the toroidal surface. (b) Gastrula cleared with Murray's Clear (MC) solution. Expression signal is detected in inner cells of the embryo. (c) Biased toward the oral pole expression covers half of the preplanula/early planula. Double arrow shows the direction of the oral–aboral (O–A) body axis. (d) Longitudinal section of the embryo through the level indicated by the white dotted line in (c). DpBra2 transcripts are visualized in the perinuclear cytoplasm of ectodermal (Ect) cells. End endoderm. Black dotted line shows the basal lamina. (e) Oral expression is slightly biased to the pole in the mature planula. (f) Longitudinal section of the planula through the level indicated by the white dotted line in (e). Expression is localized mostly in the oral ectoderm. (g) A blowup of the (f). DpBra2 transcripts are visualized in apical domains of ectodermal cells. Black arrow indicates single endodermal cell with DpBra2 expression. Black dotted line marks the basal lamina. (h) The scheme of expression patterns of DpBra2 during development.
