Figure 5
Spatial expression patterns of DpBra3 during embryonic development. (a) Broad expression domain at the end of gastrulation. White arrowhead points to the opening in the centre of the toroidal surface. (b,c) Expression forms central belt showing in the ectoderm of the preplanula. Double arrow shows the direction of the oral–aboral (O–A) body axis. (d) Expressing cells are visible as a broad central belt in the early planula. (e,f) Longitudinal sections of the larva through the levels indicated by the white dotted lines in (d). Expression is strictly ectodermal, staining is visualized mostly in basal cell domains. (g) Intense staining is visible in scattered cells in the central region of the mature larva. Weak staining is observed in the aboral endoderm. (h) Longitudinal section of the planula through the level indicated by the white dotted line in (g). Intensely stained cells are located in the ectoderm. (i,j) Blowups of the (h). Columnar and triangular bodies of expressing cells lie right above the basal lamina. Ect endoderm, End endoderm. Black dotted line marks the basal lamina. (k) The scheme of expression patterns of DpBra3 during development.
