Figure 8
Molecular phenotype of Xenopus animal caps injected with D. pumila Brachyury genes. (a,b) RT-qPCR analysis on the induction of actc1.L, myod2.S, and tubb2b.S by D. pumila Brachyury mRNAs. Data are presented as normalized fold change expression (mean ± s.d.) in experimental groups. n n-value. The number of experimental groups is 3, 3, and 2 in (a); 3, 2, and 2 in (b). ***p < 0.001. (c) Gel electrophoresis for myod.S after injection by D. pumila Brachyury mRNAs. NC negative control. L DNA ladder. Arrowhead points to 500 bp band of the DNA ladder. See non-processed gel image in Supplementary Information Fig. S4.
