Table 1 PCR and qPCR primers used in this study.

From: The evolutionary history of Brachyury genes in Hydrozoa involves duplications, divergence, and neofunctionalization

Gene

Direct primer 5' ‑> 3'

Reverse primer 5' ‑> 3'

DpBra1 in situ probe

TTGGTGGCGACAGCGAAGAA

CGGCCACGTGTTGTTTTGAATG

DpBra2 in situ probe

GAACGGAGAGGGCAAAGACAAAC

GACGGCGAATATGGGGAACAAAT

DpBra3 in situ probe

AATAATTCTTCACCGTCCAACAGG

CGCGCTTTTCGTGATAGATAGG

XlTubb2b.S (β-tubulin)

qPCR

GATCCTACCGGCAGTTACCA

TGACAGAGTCCATTGTGCCT

XlActc1.L (cardiac actin) qPCR

CTATGTGGCTTTGGACTTTGAG

GCTGTTGTAGGTAGTTTCATG GA

XlMyod1.S qPCR

AGTGACAGCCCAAATGACTC

AGAAGGGATGGTGATTACTCTC

XlEF1a qPCR

CCCTGCTGGAAGCTCTTGAC

GGACACCAGTCTCCACACGA

XlODC qPCR

GGGCTGGATCGTATCGTAGA

TGCCAGTGTGGTCTTGACAT

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