Figure 6

Taxonomic tree of antifreeze-protein-producing Collembola. A taxonomic tree for species from the four orders of Collembola (Entomobryomorpha, Poduromorpha, Symphypleona, and Neelipleona) was generated based on NCBI taxonomy. Species with and lacking TH activity are coloured in red and blue, respectively, and groups not tested are in black. Species assayed in this paper are bolded and all other species not bolded were tested by Zettel28, except Gomphiocephalus hodgsoni30 and Cryptopygus terranovus (syn. Gressittacantha terranova)53. Species with a star produced glycine-rich AFPs. Gomphiocephalus hodgsoni with a red square is a proposed cystine- and histidine-rich AFP30.