Figure 3
From: Cancer-associated fibroblast-derived exosome microRNA-21 promotes angiogenesis in multiple myeloma
Induction and identification of myeloma-associated endothelial cells. (a) CCK-8, (b) a transwell invasion test, and (c) transwell migration test were used to detect the proliferation, invasion, and migration abilities of MMECs and HUVECs. (d) qRT-PCR was used to detect the expression of the following tumor-related endothelial cell markers: TEM5 and TEM7 in MMECs and HUVECs. (e) The ability of MMECs and HUVECs to secrete VEGFA was detected by ELISA. *p < 0.05; **p < 0.01; ***p < 0.001.
