Table 1 RT-qPCR primer sequence of selected hub and reference genes and their amplification characteristics.
Primer name | Primer sequence | PCR product length (bp) | TM | PCR amplification efficiency |
|---|---|---|---|---|
IAR4 | F: GGTCCTATTATCCTTGAGATGG | 224 | 48.9 | 1.92 |
R: GTGAATAGCTCAGATGCATC | 44.3 | |||
PYD2 | F: GTGGAGACCGGCAAGATCAC | 152 | 54.2 | 1.89 |
R: CACGTTCGTGTCGGACCTCG | 58.6 | |||
TOPII | F: GAGCAAAGAAGAAGGCTCCAG | 257 | 56.3 | 1.96 |
R: CACACCAATTGCCAGAACCTC | 54.3 | |||
THY-1 | F: TATCAGCGTTCAGCAGATATG | 238 | 48.2 | 1.90 |
R: CTTGTGAGGATCATAGCCTG | 47 | |||
R: CTTCTGGAAATGCTAATC | 49 | |||
Actin | F: CCAGGGCAGAGTACGAAGAG | 153 | 51.5 | 1.96 |
R: GGAACATGGTAGACCCACC | 55 |
