Figure 1 | Scientific Reports

Figure 1

From: Analysis of the impact of DGAT1 p.M435L and p.K232A variants on pre-mRNA splicing in a full-length gene assay

Figure 1

Qualitative RT-PCR analysis of the endogenous DGAT1 splicing pattern in MAC-T cells. (A) Illustration depicting the structure of the bovine DGAT1 gene. Blue boxes and lines represent bovine DGAT1 exons and introns, respectively. Primers pairs P1-P2, P3-P4 and P5-P2 are indicated in red. (B) Gel electrophoresis of P1-P2, P3-P4 and P5-P2 RT-PCR products obtained from three different preparations of MAC-T cells. Spliced (s) and unspliced (u) products are indicated by yellow arrows and their structure is depicted on the right. (C) Genotype of MAC-T cells at positions p.232 and p.435.

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