Table 1 Description of PCR primers.
Name | Sens | Sequence | Use |
|---|---|---|---|
C1 | F | TACCGAGCTCGGATCCACACAGACAAGGACGGAGAC | DGAT1 cloning |
C2 | R | GCCCTCTAGACTCGAGCAAAGCAGTCCAACACCCACG | DGAT1 cloning |
M1 | F | GGCCTTCACCGGCCTGATGGCGCAGGTGA | p.M435L mutagenesis |
M2 | R | TCACCTGCGCCATCAGGCGGTGAAGGCC | p.M435L mutagenesis |
M3 | F | GTAGCTTTGGCAGGTAAGGCGGCCAACGGGGGAGCTG | p.K232A mutagenesis |
M4 | R | CAGCTCCCCCGTTGGCCGCCTTACCTGCCAAAGCTAC | p.K232A mutagenesis |
P1 | F | ACACAGACAAGGACGGAGAC | Qualitative DGAT1 RT-PCR |
P2 | R | GTAGTTGCCGCGGAAGAAG | Qualitative DGAT1 RT-PCR |
P3 | F | TCAAGCTGTTCTCCTACCGG | Qualitative DGAT1 RT-PCR |
P4 | R | GGGGCGAAGAGGAAGTAGTA | Qualitative DGAT1 RT-PCR |
P5 | F | CAAGTGGGCAGCCAGGAC | Qualitative DGAT1 RT-PCR |
P6 | F | CTGGCTAGCGTTTAAACTTAAGC | Qualitative pcDNA3.1 UTR RT-PCR |
P7 | R | GATCAGCGGGTTTAAACGGG | Qualitative pcDNA3.1 UTR RT-PCR |
Q1 | F | ACACAGACAAGGACGGAGAC | Quantitative DGAT1 RT-PCR |
Q2 | R | GGGTCAAAGGTTAGGGGTCA | Quantitative DGAT1 RT-PCR |
Q3 | R | TGAAGCCACTGTCAGAACTG | Quantitative DGAT1 RT-PCR |
Q4 | F | AACTACCGTGGCATCCTGAA | Quantitative DGAT1 RT-PCR |
Q5 | R | ACCGTGCGTTGCTTAAGAT | Quantitative DGAT1 RT-PCR |
Q6 | R | ACAGAGCTCCATTCACCACA | Quantitative DGAT1 RT-PCR |
Q7 | F | TCAAGCTGTTCTCCTACCGG | Quantitative DGAT1 RT-PCR |
Q8 | R | CGAGGCAGCCCTCACCAG | Quantitative DGAT1 RT-PCR |
Q9 | R | CTTACCTGCCAAAGCAGC | Quantitative DGAT1 RT-PCR |
Q10 | F | ACGTGGATCCTGAGAACTTCA | Quantitative pcAT7-Glo1 RT-PCR |
Q11 | R | TAAACGGGCCCTCTAGAGC | Quantitative pcAT7-Glo1 RT-PCR |
