Figure 2

Batch Comparability and Construct Identity Assayed by Oligonucleotide Mapping. (A) Three GMP-manufactured BNT162b2 Original DS batches were evaluated by oligonucleotide mapping. The resulting UV chromatograms are visually comparable, demonstrating process consistency. Supplementary Data Fig. 3 provides a six-segment magnification of these data. Batch 3 was made at small scale; Batches 1 and 2 were made by the same GMP process. (B) Partial oligonucleotide maps of the BNT162b2 Original, BNT162b2 Delta, and BNT162b2 Omicron mRNA DS. RNase T1-digest oligonucleotide sequences shown in black are shared by all 3 variant constructs; blue sequences are shared by BNT162b2 Original and BNT162b2 Delta constructs; green is shared by BNT162b2 Original and BNT162b2 Omicron constructs; and orange and red oligonucleotides are unique to the BNT162b2 Delta and BNT162b2 Omicron constructs, respectively. (C) Partial oligonucleotide maps of two batches of BNT162b2 Original overlaid (top pane), and one BNT162b2 Original batch overlaid with BNT162b2 Delta (bottom pane). Differences between the BNT162b2 Original and BNT162b2 Delta construct chromatograms are annotated with the oligonucleotides accounting for the difference. Blue oligonucleotides are shared by BNT162b2 Original and BNT162b2 Delta constructs; the orange oligonucleotide is unique to the BNT162b2 Delta construct. The numbers in parentheses count the number of sequence repeats in each construct sequence: red signifies the number of occurrences in the BNT162b2 Delta construct and black signifies the number of occurrences in the BNT162b2 Original construct.