Table 2 Antimicrobial activity of the ethanolic extract of hairy roots and normal plant from hypocotyl explants of Hyoscyamus muticus L. as decided by the disc diffusion method.
Microorganisms | Inhibition zones (mm) | |||
|---|---|---|---|---|
Transgenic hairy roots extract | Normal plant Extract | Positive control | Negative control | |
S. aureus (ATCC 29,213) | 18.2 ± 0.37 | 9.3 ± 0.47 | 20.0 ± 0.81 | −ve |
B. subtilis (NBRC:13,719) | 20.3 ± 0.72 | 15.6 ± 1.69 | 25.0 ± 0.81 | −ve |
P. aeruginosa (ATTC35639) | −ve | −ve | 17.0 ± 2.1 | −ve |
E. coli (ATTC11775) | 9.0 ± 0.83 | 7.0 ± 0.81 | 19.0 ± 0.81 | −ve |
K. pneumonia (ATCC 15,380) | −ve | −ve | 22.3 ± 1.6 | −ve |
C. albicans (ATTC90028) | 19.5 ± 0.91 | 9.3 ± 0.47 | 19.0 ± 0.81 | −ve |
A. terreus (NIH2624) | 14.2 ± 0.80 | 6.6 ± 0.47 | 14.6 ± 0.47 | −ve |
F. chlamydosporum (F25) | −ve | −ve | 16.3 ± 0.47 | −ve |
A. alternata (Te19) | −ve | −ve | 17.0 ± 0.81 | −ve |
M. canis (IFM 45,829) | 8.5 ± 1.20 | −ve | 16.0 ± 3.7 | −ve |