Figure 10
Biofilm prevention ability of the isolated SBSEC phages against the five bacterial strains used in this study. (a) The effects of phages vB_SbRt-pBovineB21 and vB_SbRt-pBovineS21 on total biomass of biofilm formation at 107 PFU/mL for 24 h. Control was a bacteria culture without phage. The values plotted in the box and whiskers graph using GraphPad Prism 7 revealed all points along with the minimum and maximum. Each experiment was independently undertaken in triplicate. (b) CLSM images of selected-five bacterial strains biofilm treated with the two isolated phages (107 PFU/mL) for 24 h. Green fluorescence observed using Syto 9 indicate live cells. The scale bar is 100 μM.
