Figure 5
From: Simultaneous reduction of all ORMDL proteins decreases the threshold of mast cell activation
Role of ORMDL proteins in the production of cytokines. (a) Relative mRNA expression of TNF-α, IL-6 and IL-13 in non-activated BMMCs and BMMCs activated with 1000 ng/ml TNP-BSA for 1 h. n = 6 biological replicates. (b) Concentration of secreted TNF-α, IL-6 and IL-13 cytokines measured in supernatants of non-activated BMMCs and BMMCs activated with 1000 ng/ml TNP-BSA for 5 h. n = 4 biological replicates. Data were analysed by one-way ANOVA with Tukey’s posttest (a) or Kruskal–Wallis test with Dunn’s posttest (b). *p < 0.05, **p < 0.01 and ***p < 0.001. All the results are represented as mean ± SEM. N.D.—not detected.
