Figure 6
From: Cigarette smoke extract impairs gingival epithelial barrier function
Effects of CSE on permeability of gingival epithelium to LPS and PGN. (a) Schematic illustration of three-dimensional culture of IHGE cells. (b–g) Permeability of gingival epithelial tissues (WT or with JAM1 overexpression) to FITC–P. gingivalis LPS (b, d, f) and FITC–P. gingivalis PGN (c, e, g) following exposure to CSE. Three-dimensional tissues in culture inserts with or without CSE in the upper compartment were treated with an FITC-labeled tracer. Following 3 h of incubation, transmission of the tracer from the upper to lower compartment was analyzed using spectrometry. Values are expressed as fold change relative to the control (WT cells) shown as the mean ± SD of eight technical replicates. *p < 0.05, one-trailed t test (closed testing procedure).
