Figure 8
From: Cigarette smoke extract impairs gingival epithelial barrier function
Vitamin C induces JAM1 expression in IHGE cells. (a) Relative levels of JAM1 mRNA expression in vitamin C-treated IHGE cells. IHGE cells were treated with vitamin C at the indicated concentration for 24 h, then sampled for a qRT-PCR assay. Results are expressed as fold change relative to no administration with five technical replicates. The significance of differences was evaluated using a two-tailed t test. (b) IHGE cells were treated with vitamin C at the indicted concentration. Following 24 h of incubation, cells were analyzed by immunoblotting with the indicated antibodies. Full-length blots are shown in Supplementary Figure 7. (c) IHGE cells were treated with 2.5 mM vitamin C and incubated for 1 h, then treated with the combination of CSE and vitamin C, and incubated for 1 h. The cells were then fixed, stained with DAPI (cyan) and mouse monoclonal anti-JAM1 (FITC; green), and analyzed using confocal microscopy. Scale bars, 10 μm.
