Figure 3 | Scientific Reports

Figure 3

From: MTAP-ANRIL gene fusion promotes melanoma epithelial-mesenchymal transition-like process by activating the JNK and p38 signaling pathways

Figure 3

Overexpression of MTAP-ANRIL regulated the expression of metastasis and proliferation-related genes. (A, B) Real-time PCR analysis of metastasis-related gene (E-cadherin and N-cadherin) expression and proliferation-related gene (Cyclin B1 and Cyclin D1) expression in A375 (A) and A875 (B) melanoma cells after transfection with the MTAP-ANRIL plasmid. (C) Real-time PCR analysis of EMT-related gene (SNAIL1 and SNAIL2) expression in A375 (left) and A875 (right) melanoma cells after transfection with the MTAP-ANRIL plasmid. (D) Western blot analysis of metastasis-related gene (E-cadherin and N-cadherin) expression, EMT-related gene (SNAIL1 and SNAIL2) and proliferation-related gene (Cyclin B1 and Cyclin D1) expression in melanoma cells after transfection with the MTAP-ANRIL plasmid. The data are presented as the mean ± SD values. *P < 0.01.

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