Figure 2
From: Structural and functional analysis of a plant nucleolar RNA chaperone-like protein
SEC-SAXS analysis of NURC1FL and truncated NURC1 proteins. (a) Representation of NURC1FL and NURC1 proteins with truncations at positions 160, 140 and 53. The putative RNA recognition motif (RRM) is represented as dark grey box. (b) SEC chromatogram showing the average intensity (black line) and the Rg predicted values for the analyzed range of frames (purple spheres) of the NURC1 full-length protein. The loaded sample concentration was 18 mg/ml. (c) Guinier fit (red line) of the experimental data (black spheres) on the top and standardized residuals on the bottom. (d) Dimensionless Krakty plot. (e) P(r) versus r profile.
