Figure 4
From: Structural and functional analysis of a plant nucleolar RNA chaperone-like protein
The RRM structure and RNPs aromatic residues are highly conserved. NURC1 AlphaFold model Q9LZ65 (red), NIFK AlphaFold model Q9BYG3 (grey) and Nop15 CryoEM structure PDB 3JCT (light blue) represented as cartoons (top left) and RRMs (top right). The superimposition of the three models was performed in Pymol by using the alignment plugin (align, outlier rejection, 20 cycles and a cutoff of 2). The Nop15 structure was used as target. The final RMSD values for NURC1 and NIFK were 0.595 and 0.776, respectively. Sequence alignment was performed using Clustal Omega for all three proteins (bottom). RNPs are highlighted in sky blue and conserved amino acids are inside boxes. The RRM secondary structure is displayed on the bottom as a cartoon where β-sheets are represented as orange arrows and α-helices as green rods.
